The Gadsden flag, that includes a rattlesnake and the phrase "Don't Tread on Me

https://daltonhmgr084.terracolumn.com/posts/a-collector-s-dream-the-fascinating-world-of-civil-war-flags-4

The Gadsden flag, that includes a rattlesnake and the word "Don't Tread on Me," become adopted with the aid of some Confederate units as a symbol of resistance in opposition to perceived oppression.